Isolation and characterisation of the major outer membrane protein of Erwinia carotovora
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چکیده
منابع مشابه
Isolation of the outer membrane and characterization of the major outer membrane protein from Spirochaeta aurantia.
The outer membrane of Spirochaeta aurantia was isolated after cells were extracted with sodium lauryl sarcosinate and was subsequently purified by differential centrifugation and KBr isopycnic gradient centrifugation. The purified outer membrane was obtained in the form of carotenoid-containing vesicles. Four protein species with apparent molecular weights of 26,000 (26K), 36.5K, 41K, and 48.5K...
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The lipopolysaccharide (LPS) from Erwinia carotovora, strain IFO 14082 has been isolated and found to contain 0glucose, L-glycero-o-manno-heptose, 2-acetamido-2-deoxy-o-glucose and 3-deoxy-o-manno-octulosonic acid. The main fatty acid components of lipid-A of the LPS are CI2:0, 3-0H-CI2:0, CI4:0, 3-0H-CI4:0 and CI6:0. The O-specific side chain (PS), obtained from the LPS, shows the presence of ...
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Polygalacturonase of Erwinia carotovora.
The polygalacturonase, clearly differentiated from polygalacturonic acid trans-eliminase, produced in the culture fluid of Erwinia carotovora was purified 56-fold by means of acetone precipitation, acetate butfer extraction, and two successive treatments by chromatography on carboxymethyl cellulose. The purified enzyme is specific for nonmethoxylated polygalacturonic acid. It shows an optimum p...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Biomembranes
سال: 2001
ISSN: 0005-2736
DOI: 10.1016/s0005-2736(01)00387-x